9N2V
Designed anti-OSM Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2024-09-04 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.03321 |
| Spacegroup name | P 41 |
| Unit cell lengths | 117.890, 117.890, 99.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.550 - 1.970 |
| R-factor | 0.1755 |
| Rwork | 0.174 |
| R-free | 0.20220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.589 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.550 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.154 | 1.620 |
| Rmeas | 0.160 | 1.684 |
| Rpim | 0.043 | 0.457 |
| Total number of observations | 1308844 | 63068 |
| Number of reflections | 95394 | 4704 |
| <I/σ(I)> | 16.1 | 2.1 |
| Completeness [%] | 99.5 | |
| Redundancy | 13.7 | 13.4 |
| CC(1/2) | 0.999 | 0.745 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.2 M sodium chloride, 0.1 M imidazole, pH 8.0, 0.4 M ammonium phosphate, 1.6 M potassium phosphate dibasic |
