9N2N

Structure of M tuberculosis NapA bound to 20mer AT rich DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	92.031, 92.031, 107.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.430 - 3.870
R-factor	0.3066
Rwork	0.300
R-free	0.32780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.612
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.420	4.080
High resolution limit [Å]	3.870	3.870
Rpim	0.022	1.550
Number of reflections	2285	325
<I/σ(I)>	13	0.2
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)	0.999	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	7.5% MPD, MES buffer