9N1S

Crystal structure of the Transport and Golgi Organization protein 2 Homolog (TANGO2) tetragonal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-14
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 41 21 2
Unit cell lengths	49.895, 49.895, 241.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.120 - 2.300
R-factor	0.2072
Rwork	0.205
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.575
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_5533: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.120	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.103	1.066
Rmeas	0.113	1.167
Rpim	0.046	0.468
Total number of observations	82398	8441
Number of reflections	14036	1399
<I/σ(I)>	10.8	1.8
Completeness [%]	96.4
Redundancy	5.9	6
CC(1/2)	0.998	0.781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	291	Berkeley Screen B8: 20% (w/v) PEG 3350, 70 mM Bis-Tris Propane, 30 mM Citric Acid pH 7.6 , TANGO2 at 5.5 mg/mL. Plate 19608 well B8 drop 1, Puck: PSL-2010, Cryo: 80% (v/v) crystallant and 20% (v/v) PEG 200