9MZC

anti-IL6 designed Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2024-09-12
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	50.240, 64.820, 139.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.260 - 1.580
R-factor	0.1766
Rwork	0.175
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.913
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.470	1.600
High resolution limit [Å]	1.580	1.580
Rmerge	0.167	1.070
Rmeas	0.180	1.153
Rpim	0.068	0.426
Total number of observations	427878	20635
Number of reflections	62430	2909
<I/σ(I)>	12.4	2
Completeness [%]	98.1
Redundancy	6.9	7.1
CC(1/2)	0.995	0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.09M NPS, 0.1M BS1 pH6.5, 50 % v/v, PM2 (Polymer)