9MZB

X-ray crystallographic structure of Orf9b Apo Homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2023-10-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.116
Spacegroup name	P 31 2 1
Unit cell lengths	75.280, 75.280, 80.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.640 - 2.800
R-factor	0.2914
Rwork	0.286
R-free	0.34140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.843
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.640	2.910
High resolution limit [Å]	2.800	2.800
Rmerge	0.062	1.187
Rmeas	0.069	1.414
Rpim	0.031	0.757
Number of reflections	12518	1367
<I/σ(I)>	11.49	0.93
Completeness [%]	99.7	98.06
Redundancy	4.9	3.3
CC(1/2)	0.998	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1.2M Sodium Phosphate, 0.8M Potassium Phosphate, 0.2M Lithium Sulfate, and 0.1M Glycine pH 10.5