9MZ9

Crystal Structure of 19b Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	46.693, 83.729, 136.628
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.930 - 2.100
R-factor	0.2172
Rwork	0.216
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.584
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.930	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	32061	2611
<I/σ(I)>	10.5
Completeness [%]	100.0
Redundancy	12.7
CC(1/2)	0.997	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Magnesium acetate tetrahydrate, 20% w/v Polyethylene glycol 3,350, pH 7.9