9MZ7

Crystal Structure of 19b Fab bound to the third variable (V3) loop peptide from the HIV-1 92BR020 envelope (Env) glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	46.572, 81.454, 134.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.780 - 1.940
R-factor	0.2223
Rwork	0.220
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.535
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.780	1.990
High resolution limit [Å]	1.940	1.940
Rmerge	0.107
Number of reflections	37735	2569
<I/σ(I)>	11
Completeness [%]	98.6
Redundancy	5.8
CC(1/2)	0.997	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.18 M Ammonium sulfate, 0.09 M Sodium acetate trihydrate pH 4.6, 27% w/v Polyethylene glycol monomethyl ether 2,000 , 10% v/v glycerol