9MYP

Structure of Patiria miniata Hop1 chromatin binding region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2827
Spacegroup name	P 21 21 21
Unit cell lengths	38.654, 106.955, 131.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.950 - 1.840
R-factor	0.1997
Rwork	0.198
R-free	0.23540
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.950	1.880
High resolution limit [Å]	1.840	1.840
Rmerge	0.067
Number of reflections	47903	2772
<I/σ(I)>	14.7
Completeness [%]	98.9
Redundancy	3.2
CC(1/2)	0.997	0.454

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Bis Tris pH 6.5, 0.1 M potassium thiocyanate, and 17% PEG 3350