9MYL
Fertilization IZUMO1 Protein Ectodomain in Complex with Anti-sperm Antibody OBF13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-07-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 |
| Unit cell lengths | 81.069, 83.208, 92.605 |
| Unit cell angles | 82.85, 89.72, 71.40 |
Refinement procedure
| Resolution | 40.062 - 3.177 |
| R-factor | 0.2145 |
| Rwork | 0.212 |
| R-free | 0.26330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.032 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.062 | 3.270 |
| High resolution limit [Å] | 3.177 | 3.180 |
| Number of reflections | 34758 | 2932 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 90.7 | |
| Redundancy | 2.2 | |
| CC(1/2) | 0.994 | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 200 mM L-Proline, 100 mM HEPES pH 7.4, 9% PEG 3,350 |
