9MYE

C28 Polymerase Incorporating RNA, n+1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	77.970, 102.340, 112.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.030 - 2.220
Rwork	0.241
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.030	2.299
High resolution limit [Å]	2.220	2.220
Rmerge	0.078	0.817
Rmeas	0.110	1.155
Rpim	0.078	0.817
Number of reflections	45059	4429
<I/σ(I)>	5.21	0.9
Completeness [%]	99.2	99.84
Redundancy	2	2
CC(1/2)	0.987	0.308

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	MORPHEUS II G10 [12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol, 0.02M of each Amino-acid II, 0.1M GlyGly/AMPD pH 8.5]