9MYA
Crystal structure of unliganded retro-aldolase RA95 (277 K)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 277 |
| Detector technology | PIXEL |
| Collection date | 2023-04-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.992, 65.152, 44.377 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.000 - 1.890 |
| R-factor | 0.1619 |
| Rwork | 0.160 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.820 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 1.980 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 23151 | 1137 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 98.6 | 97.3 |
| Redundancy | 6.4 | 6.7 |
| CC(1/2) | 0.988 | 0.576 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 278 | 0.1 M sodium acetate pH 5.2 3.1 M NaCl 7 mg/mL protein |
