9MVY
Crystal structure of ZMET2 in complex with unmethylated CTG DNA and a histone H3Kc9me2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-09-08 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.515, 277.296, 64.479 |
| Unit cell angles | 90.00, 97.26, 90.00 |
Refinement procedure
| Resolution | 48.400 - 2.710 |
| R-factor | 0.2282 |
| Rwork | 0.227 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.619 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 2.780 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.147 | |
| Number of reflections | 59203 | 3959 |
| <I/σ(I)> | 5.04 | |
| Completeness [%] | 98.0 | 91.54 |
| Redundancy | 2.8 | 2.8 |
| CC(1/2) | 0.973 | 0.199 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M magnesium acetate, 0.1 M sodium cacodylate pH 6.5, 15% w/v polyethylene glycol 6,000 |
