9MVG
Structure of SciW variant L66A bound to the Rhs1 transmembrane domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.1808 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.670, 52.020, 177.450 |
| Unit cell angles | 90.00, 99.77, 90.00 |
Refinement procedure
| Resolution | 45.160 - 3.050 |
| R-factor | 0.2635 |
| Rwork | 0.262 |
| R-free | 0.29930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.670 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.160 | 3.260 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.151 | 1.948 |
| Rpim | 0.064 | 0.816 |
| Number of reflections | 15847 | 15847 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.6 | 6.6 |
| CC(1/2) | 0.996 | 0.492 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 20mg/mL protein, 0.1 M MES pH 6, 0.2 M MgCl2, and 20% (v/v) PEG 6000 |
