9MUZ

anti-IL6 designed Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2024-04-22
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	94.017, 147.329, 61.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.660 - 1.710
R-factor	0.1675
Rwork	0.166
R-free	0.19610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.644
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	147.330	1.740
High resolution limit [Å]	1.710	1.710
Rmerge	0.108	0.861
Rmeas	0.116	0.942
Rpim	0.043	0.378
Total number of observations	644863	26991
Number of reflections	91871	4507
<I/σ(I)>	11.3	1.9
Completeness [%]	100.0
Redundancy	7	6
CC(1/2)	0.997	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.2 M Sodium Potassium phosphate, 20 % v/v Polyethylene glycol 3,350