9MUX

Structure of UBR1-RWA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2024-11-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97741
Spacegroup name	P 1 21 1
Unit cell lengths	27.091, 43.809, 27.085
Unit cell angles	90.00, 111.27, 90.00

Refinement procedure

Resolution	25.250 - 1.290
R-factor	0.1421
Rwork	0.142
R-free	0.15070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.928
Data reduction software	HKL-2000
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.310
High resolution limit [Å]	1.290	1.290
Rmerge	0.042	0.104
Rpim	0.021	0.056
Number of reflections	14074	298
<I/σ(I)>	19.471	19.471
Completeness [%]	94.2	82.4
Redundancy	4.7	4.3
CC(1/2)		0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	283.15	0.1M HEPES buffer 25%PEG3350