9MUV

Oxidized state of a turn-on thiol-disulfide redox biosensor with a fluorescence-lifetime readout

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2023-05-02
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	52.550, 140.430, 130.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.110 - 2.200
R-factor	0.1924
Rwork	0.189
R-free	0.23430
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	0.466
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.110	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	24648	2164
<I/σ(I)>	10.81
Completeness [%]	98.3
Redundancy	6.9
CC(1/2)	0.997	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	5% Tacsimate (pH 4.0), 16% PEG 3350, 0.1 M sodium acetate (pH 4.8), 10 mM oxidized DTT