9MUU
Oxidized state of a turn-off thiol-disulfide redox biosensor with a fluorescence-lifetime readout
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-06-21 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.270, 159.610, 49.600 |
| Unit cell angles | 90.00, 94.23, 90.00 |
Refinement procedure
| Resolution | 30.550 - 2.150 |
| R-factor | 0.2112 |
| Rwork | 0.209 |
| R-free | 0.24360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.471 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.550 | 2.227 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 34351 | 3378 |
| <I/σ(I)> | 7.17 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.993 | 0.252 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.5% Tacsimate (pH 4.0), 0.2 M sodium acetate (pH 5.0), 17.5% PEG 3350, 10 mM oxidized DTT |
