9MUT

Reduced state of a turn-on thiol-disulfide redox biosensor with a fluorescence-lifetime readout

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2023-06-04
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	49.950, 140.840, 129.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.680 - 2.200
Rwork	0.199
R-free	0.24510
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	1.227
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.680	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	23498	2310
<I/σ(I)>	12.41
Completeness [%]	99.2
Redundancy	5.1
CC(1/2)	0.998	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	5% Tacsimate (pH 4.0), 16% PEG 3350, 0.1 M sodium acetate (pH 4.8), 10 mM TCEP (pH 7.0)