9MUS

Reduced state of a turn-off thiol-disulfide redox biosensor with a fluorescence-lifetime readout

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2023-04-25
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	52.800, 140.180, 130.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.850 - 2.000
Rwork	0.186
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	1.171
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.850	2.072
High resolution limit [Å]	2.000	2.000
Number of reflections	32802	14547
<I/σ(I)>	11.28
Completeness [%]	99.1
Redundancy	9.1
CC(1/2)	0.997	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2.5% glycerol, 5% Tacsimate (pH 4.0), 0.1 M sodium acetate (pH 5.2), 16% PEG 3350, 10 mM TCEP (pH ~7.0)