9MUK
RlmR 23S rRNA methyltransferase from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980098 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 221.840, 62.152, 98.041 |
| Unit cell angles | 90.00, 114.21, 90.00 |
Refinement procedure
| Resolution | 101.168 - 1.973 |
| R-factor | 0.2058 |
| Rwork | 0.205 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.231 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.170 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.143 | 2.380 |
| Rmeas | 0.149 | 2.480 |
| Rpim | 0.041 | 0.692 |
| Total number of observations | 1114946 | 155654 |
| Number of reflections | 86088 | 12513 |
| <I/σ(I)> | 9.8 | 1.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 13 | 12.4 |
| CC(1/2) | 0.998 | 0.527 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4 | 292 | Na-Citrate 0.1M, pH4, PEG6000 20%, LiCl 1M |
