9MUG

Structure of Schistosoma mansoni Hop1 chromatin binding region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.2827
Spacegroup name	P 21 21 21
Unit cell lengths	33.032, 64.959, 65.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.630 - 1.450
R-factor	0.1303
Rwork	0.129
R-free	0.15830
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.382
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.960	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.080	0.458
Number of reflections	135024	605
<I/σ(I)>	30.9
Completeness [%]	94.4
Redundancy	5.6
CC(1/2)	0.997	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M MES pH 6.0, 0.1 M sodium acetate, and 25% PEG 3350