9MSW

anti-EGFR DyAb designed FAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2024-04-29
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 32
Unit cell lengths	77.906, 77.906, 202.295
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.730 - 2.100
R-factor	0.1815
Rwork	0.179
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.643
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	202.290	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.170	1.127
Rmeas	0.184	1.217
Rpim	0.070	0.457
Total number of observations	550321	30932
Number of reflections	79881	4481
<I/σ(I)>	9.9	2
Completeness [%]	100.0
Redundancy	6.9	6.9
CC(1/2)	0.996	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	291	0.1M sodium acetate pH 4.6, 0.2M sodium bromide, 20% w/v PEG 3350