9MSC

Structure of Hepatitis C Virus Envelope Glycoprotein HCV-1 E2ecto from genotype 1a bound to neutralizing antibody RM5-16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	72.709, 72.709, 564.779
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.640 - 3.390
R-factor	0.2814
Rwork	0.278
R-free	0.31650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.661
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.500
High resolution limit [Å]	3.390	3.390
Number of reflections	24462	1630
<I/σ(I)>	6.64
Completeness [%]	95.8
Redundancy	4.3
CC(1/2)	0.990	0.388

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.3 M magnesium chloride, 15% w/v PEG3350