9MS9

Structure of HCV neutralizing antibody RM5-16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	43.072, 70.168, 161.068
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.640 - 1.460
R-factor	0.1446
Rwork	0.143
R-free	0.17480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.640	1.510
High resolution limit [Å]	1.460	1.460
Number of reflections	85214	4221
<I/σ(I)>	24.13
Completeness [%]	99.4
Redundancy	13.1
CC(1/2)	0.995	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium acetate, pH 4.5, 0.2 M lithium sulfate, 28% w/v PEG8000