9MS5

Crystal structure of an expansin (Ss_EXLX1) from Streptomyces sp.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	68.396, 101.634, 116.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.660 - 1.450
R-factor	0.1687
Rwork	0.167
R-free	0.20020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.785
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.700	1.487
High resolution limit [Å]	1.450	1.450
Rmeas	0.141
Rpim	0.071	0.957
Number of reflections	140101	7005
<I/σ(I)>	10.5	1.9
Completeness [%]	97.0
Redundancy	7.5
CC(1/2)	0.998	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Bis-Tris: HCl, pH 6.5 , 20 % (w/v) PEG MME 5000