9MRZ
Structure of HCV broadly neutralizing antibody RM1-73
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-09-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.889, 73.329, 99.685 |
| Unit cell angles | 90.00, 94.23, 90.00 |
Refinement procedure
| Resolution | 44.790 - 2.030 |
| R-factor | 0.171 |
| Rwork | 0.170 |
| R-free | 0.19590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.669 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21rc1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.790 | 3.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.330 | |
| Number of reflections | 80829 | 2524 |
| <I/σ(I)> | 26.43 | |
| Completeness [%] | 97.7 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.990 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M CHES, pH 9.5, 0.2 M sodium chloride, 1.26 M ammonium sulfate |
