9MRW
Functional Implications of Hexameric Dynamics in SARS-CoV-2 Nsp15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2023-02-27 |
| Detector | PSI JUNGFRAU 4M |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 63 |
| Unit cell lengths | 154.200, 154.200, 116.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.640 - 3.000 |
| R-factor | 0.16867 |
| Rwork | 0.167 |
| R-free | 0.19318 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.929 |
| Data reduction software | CrystFEL (v11.1) |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (CCP4-v9.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 31487 | 3119 |
| <I/σ(I)> | 6.8 | 1.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 420 | |
| CC(1/2) | 0.980 | 0.270 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 298 | 100 mM HEPES-NaOH, pH 7.5, 200 mM calcium acetate, 8% (w/v) PEG 8000 |
