9MRJ

Neisseria meningitidis factor H binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979323
Spacegroup name	C 2 2 21
Unit cell lengths	37.713, 89.536, 160.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.970 - 1.880
R-factor	0.1902
Rwork	0.189
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.863
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.970	1.980
High resolution limit [Å]	1.880	1.880
Rmerge	0.480	1.996
Rmeas	0.502	2.085
Rpim	0.146	0.596
Total number of observations	264599	39178
Number of reflections	22717	3251
<I/σ(I)>	5.6	2
Completeness [%]	100.0
Redundancy	11.6	12.1
CC(1/2)	0.979	0.414

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294.15	0.1M Tris pH 8.5, 2.5M Ammonium sulfate