9MRF
De novo designed apolar residue motif transmembrane helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 39.599, 48.745, 29.756 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.400 - 2.000 |
| R-factor | 0.2157 |
| Rwork | 0.214 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.166 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.084 | |
| Rpim | 0.034 | |
| Number of reflections | 4159 | 200 |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 98.2 | |
| Redundancy | 6 | 5.2 |
| CC(1/2) | 0.981 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7.5 | 293.15 | 0.01M HEPES pH7.5, 3.5M 1,6-Hexanediol |
