9MPN
BRD3-BD1 A128T mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.436, 126.598, 70.377 |
| Unit cell angles | 90.00, 126.97, 90.00 |
Refinement procedure
| Resolution | 42.720 - 1.600 |
| R-factor | 0.1828 |
| Rwork | 0.182 |
| R-free | 0.20980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.905 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.720 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.091 | |
| Number of reflections | 149985 | 3623 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 96.8 | |
| Redundancy | 7 | |
| CC(1/2) | 0.997 | 0.653 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Calcium chloride dihydrate, 0.1 M BIS-Tris pH 5.5, 45 % v/v MPD |
