9MPB

Crystal structure of the HIV V2-apex-targeting antibody RM038, derived from macaque ApexGT6 immunization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	181.251, 66.512, 53.405
Unit cell angles	90.00, 103.18, 90.00

Refinement procedure

Resolution	41.580 - 1.980
R-factor	0.1873
Rwork	0.185
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	1.653
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.980	1.980
Number of reflections	40445	1989
<I/σ(I)>	28.72
Completeness [%]	94.1
Redundancy	5.9
CC(1/2)	0.999	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Imidazole pH 8.0 1.0 M Sodium citrate