9MP2
Fatty Acid Binding Protein 4 (FABP4) Complexed with Perfluorohexadecanoic Acid (PFHxDA)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-20 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.217, 53.564, 75.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.610 - 1.350 |
| R-factor | 0.1798 |
| Rwork | 0.178 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.099 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.610 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.046 | 0.797 |
| Rmeas | 0.049 | 0.885 |
| Rpim | 0.016 | 0.379 |
| Total number of observations | 250919 | 7717 |
| Number of reflections | 29408 | 1430 |
| <I/σ(I)> | 25.5 | 2.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 8.5 | 5.4 |
| CC(1/2) | 1.000 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.55-1.6 M sodium citrate |
