9MOJ
Saccharolobus solfataricus GINS tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 180.236, 180.236, 51.203 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.220 - 2.300 |
| R-factor | 0.2211 |
| Rwork | 0.220 |
| R-free | 0.24280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.386 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 1.091 |
| Rmeas | 0.085 | 1.116 |
| Number of reflections | 42463 | 1957 |
| <I/σ(I)> | 34.5 | 1 |
| Completeness [%] | 99.2 | 91.5 |
| Redundancy | 37.3 | 19 |
| CC(1/2) | 1.000 | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.18 M tri-Ammonium citrate 20% (w/v) PEG 3350 |
