9MNO
Structure of the TelA-associated type VII secretion system chaperone LcpA in complex with the chaperone binding site of TelA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.822, 54.855, 119.922 |
| Unit cell angles | 99.94, 96.42, 90.78 |
Refinement procedure
| Resolution | 30.870 - 1.940 |
| R-factor | 0.2062 |
| Rwork | 0.204 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.600 |
| Data reduction software | AutoProcess |
| Data scaling software | Aimless |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.870 | 2.040 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.063 | 0.824 |
| Rmeas | 0.090 | 1.165 |
| Rpim | 0.063 | 0.824 |
| Number of reflections | 68651 | 9922 |
| <I/σ(I)> | 8.2 | 1.2 |
| Completeness [%] | 97.9 | 96.9 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.998 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.2 M ammonium tartrate dibasic, 20% PEG 3350 |
