9MMI

Myo-inositol-1(or 4)-monophosphatase that can perform essential dephosphorylation step to facilitate riboflavin biosynthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	65.190, 72.914, 119.245
Unit cell angles	90.00, 90.03, 90.00

Refinement procedure

Resolution	37.680 - 1.750
R-factor	0.1827
Rwork	0.182
R-free	0.20870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.252
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.680	1.780
High resolution limit [Å]	1.750	1.750
Rpim	0.034	0.410
Number of reflections	110871	5264
<I/σ(I)>	11.3	2
Completeness [%]	98.5
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.75 mM HEPES pH 7.5, 70% MPD