9MMH
HFP (histidine family phosphatase) that catalyzes essential dephosphorylation for riboflavin biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.015, 110.015, 256.654 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.020 - 2.040 |
| R-factor | 0.1906 |
| Rwork | 0.190 |
| R-free | 0.22520 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.281 |
| Phasing software | AutoSol (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.020 | 2.070 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rpim | 0.034 | 0.637 |
| Number of reflections | 115401 | 5580 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Tris-HCl pH 8.5, 2 M Ammonium Sulfate |
