9MLW
Crosslinked complex of ketosynthase FabB mutant FabBG107M and acyl carrier protein AcpP from E.coli with C8 crosslinker
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-18 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.410, 98.180, 78.000 |
| Unit cell angles | 90.00, 109.25, 90.00 |
Refinement procedure
| Resolution | 73.720 - 2.210 |
| Rwork | 0.191 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (v5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.720 | 9.070 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.117 | 0.617 |
| Number of reflections | 46000 | 3586 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.4 | 3.1 |
| CC(1/2) | 0.990 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | Sodium Cacodylate pH 6.0, 28% PEG8K, NaCH3COO |
