9MKL
canavanyl-tRNAArg deacylase (CtdA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-22 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.946, 87.604, 98.290 |
| Unit cell angles | 63.57, 82.13, 87.40 |
Refinement procedure
| Resolution | 21.880 - 1.980 |
| R-factor | 0.1843 |
| Rwork | 0.184 |
| R-free | 0.22970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.805 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.880 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 146681 | 3868 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 97.6 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.991 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M ammonium citrate dibasic, 20% PEG 3350 |
