9MKA

Gallid alphaherpesvirus-1 large tegument protein NLS 1 in complex with Importin alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95374
Spacegroup name	P 21 21 21
Unit cell lengths	78.850, 90.110, 100.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.050 - 2.500
R-factor	0.1719
Rwork	0.170
R-free	0.20830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.554
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.060	2.600
High resolution limit [Å]	2.500	2.500
Rpim	0.029	0.121
Number of reflections	25365	2823
<I/σ(I)>	17.7
Completeness [%]	100.0
Redundancy	6.8
CC(1/2)	0.998	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296	0.6 M sodium citrate, 0.1 M HEPES