9MK4
Crystal structure of the VRC01-class antibody 12A01 derived from GT1.1 vaccination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 43 |
| Unit cell lengths | 73.221, 73.221, 274.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.550 - 3.000 |
| R-factor | 0.2434 |
| Rwork | 0.239 |
| R-free | 0.29420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.424 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 28654 | 1477 |
| <I/σ(I)> | 19.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.5 | |
| CC(1/2) | 0.990 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50% PEG-200, 0.2 M Magnesium chloride, 0.1M cacodylate pH 6.5, final pH 6.6 |
