9MJU

Crystal structure of WRN helicase with allosteric fragment 1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	95
Detector technology	PIXEL
Collection date	2022-06-22
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	58.076, 58.500, 130.864
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.410 - 1.579
R-factor	0.2135
Rwork	0.211
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.020
Data reduction software	autoPROC
Data scaling software	autoPROC ((Version 1.1.7))
Refinement software	BUSTER (2.11.8 (10-JUL-2024))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.460	1.610
High resolution limit [Å]	1.579	1.580
Number of reflections	61984	3069
<I/σ(I)>	15.1
Completeness [%]	100.0
Redundancy	8.2
CC(1/2)	0.999	0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% v/v PEG6000, 0.1 M HEPES, pH 6.25-6.5