9MJS

Crystal structure of WRN helicase with bound AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	95
Detector technology	PIXEL
Collection date	2022-03-15
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	104.202, 93.737, 53.782
Unit cell angles	90.00, 104.44, 90.00

Refinement procedure

Resolution	45.410 - 1.840
R-factor	0.19157
Rwork	0.189
R-free	0.22518
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.380
Data reduction software	autoPROC
Data scaling software	autoPROC ((Version 1.1.7))
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.410	1.870
High resolution limit [Å]	1.840	1.840
Number of reflections	41646	2092
<I/σ(I)>	8.2
Completeness [%]	96.3	96.2
Redundancy	2.8	2.9
CC(1/2)	0.997	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	30% Jeffamine ED 2003, 0.2 M NaCl, 0.2 M NDSB-201, 0.1 M MES