9MJI

Crystal structure of the VRC01-class antibody 9C09 derived from GT1.1 vaccination

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	P 21 21 21
Unit cell lengths	70.805, 82.371, 162.921
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.780 - 2.400
R-factor	0.2342
Rwork	0.232
R-free	0.27850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.494
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.780	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	38042	1887
<I/σ(I)>	8.2
Completeness [%]	100.0
Redundancy	6.8
CC(1/2)	0.987	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2 M potassium fluoride, pH 7.2