9MJD

Crystal structure of the VRC01-class antibody 3G08

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	P 32
Unit cell lengths	107.934, 107.934, 223.563
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.590 - 3.500
R-factor	0.3084
Rwork	0.306
R-free	0.34260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.390
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.490	3.490
Number of reflections	36692	1811
<I/σ(I)>	2.8
Completeness [%]	99.7
Redundancy	9.6
CC(1/2)	0.990	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.0 M sodium/potassium tartrate, 0.2 M lithium sulfate, 0.1 M CHES pH 9.5