9MJ6

Crystal structure of the VRC01-class antibody 7A03 derived from GT1.1 vaccination

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	P 1 21 1
Unit cell lengths	50.483, 137.866, 62.983
Unit cell angles	90.00, 108.30, 90.00

Refinement procedure

Resolution	34.470 - 3.060
R-factor	0.2672
Rwork	0.264
R-free	0.32360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.478
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.470	3.110
High resolution limit [Å]	3.060	3.060
Number of reflections	15508	3080
<I/σ(I)>	3.37
Completeness [%]	99.8
Redundancy	6.8
CC(1/2)	0.910	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% PEG-1000, 0.2 M Lithium sulfate, phosphate-citrate pH 4.2