9MIK

Gallid alphaherpesvirus-1 large tegument protein bipartite NLS1 in complex with Importin alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95374
Spacegroup name	P 21 21 21
Unit cell lengths	78.087, 89.924, 99.203
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.960 - 2.600
R-factor	0.1771
Rwork	0.175
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.554
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.960	2.720
High resolution limit [Å]	2.600	2.600
Rpim	0.038	0.216
Number of reflections	21815	2627
<I/σ(I)>	13.5
Completeness [%]	99.0
Redundancy	5.8
CC(1/2)	0.997	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296	0.6 M sodium citrate, 0.1 M HEPES