9MIC

Crystal structure of the VRC01-class antibody 4D01, derived from GT1.1 vaccination, in complex with eOD-GT8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	P 21 21 21
Unit cell lengths	53.435, 71.291, 219.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.900 - 1.970
R-factor	0.2231
Rwork	0.222
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.012
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.900	2.000
High resolution limit [Å]	1.970	1.970
Number of reflections	60382	2805
<I/σ(I)>	5.8
Completeness [%]	99.9
Redundancy	12.9
CC(1/2)	0.990	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.2 M sodium dihydrogen phosphate, 0.1M CAPS, 0.8M di-potassium hydrogen phosphate, 25% glycerol, 0.2M lithium sulfate