9MHO
The structure of Zcp with nickel bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.809, 94.214, 128.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.200 - 2.300 |
| R-factor | 0.2218 |
| Rwork | 0.219 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9mhi |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.600 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.200 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 30805 | 2144 |
| <I/σ(I)> | 17.1 | 0.6 |
| Completeness [%] | 99.6 | 95.5 |
| Redundancy | 12.8 | 12.4 |
| CC(1/2) | 1.000 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% v/v tacsimate, pH 5.0, and 20% w/v polyethylene glycol 3,350 |
