9MHN
The structure of Zcp with cobalt bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.037, 94.473, 129.141 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.300 - 2.590 |
| R-factor | 0.2516 |
| Rwork | 0.248 |
| R-free | 0.28290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.516 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.300 | 2.630 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Number of reflections | 22278 | 1127 |
| <I/σ(I)> | 4.8 | 0.3 |
| Completeness [%] | 97.6 | 68.7 |
| Redundancy | 12.3 | 9.4 |
| CC(1/2) | 0.980 | 0.220 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% v/v tacsimate, pH 5.0, and 20% w/v polyethylene glycol 3,350 |
