9MHM

The structure of Zcp with copper bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.3808
Spacegroup name	P 21 21 21
Unit cell lengths	55.958, 93.269, 127.764
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.980 - 2.280
R-factor	0.2011
Rwork	0.198
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.584
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.980	2.330
High resolution limit [Å]	2.270	2.270
Number of reflections	29073	1157
<I/σ(I)>	9.6	0.4
Completeness [%]	92.2	51
Redundancy	6	4.2
CC(1/2)	1.000	0.220

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	8% v/v tacsimate, pH 5.0, and 20% w/v polyethylene glycol 3,350